Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Uncovering the link between the SpnIII restriction modification system and LuxS in Streptococcus pneumoniae meningitis isolates

doi: 10.3389/fcimb.2023.1177857

Figure Lengend Snippet: S. pneumoniae strains and oligonucleotide primers used in this study.

Article Snippet: The wildtype S. pneumoniae strains used in this study ( ) were provided to us by the German National Reference Center for Streptococci (Aachen) ( ).

Techniques: Sequencing, Mutagenesis

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Uncovering the link between the SpnIII restriction modification system and LuxS in Streptococcus pneumoniae meningitis isolates

doi: 10.3389/fcimb.2023.1177857

Figure Lengend Snippet: spnIII allele frequencies of 60B and 60CSF recovered post intranasal murine infection. Mice were intranasally challenged with 10 8 CFU S. pneumoniae serotype 15C ST8711 blood isolate (60B) or CSF isolate (60CSF). At 24 h, mice from each group were humanely euthanized and pneumococci in the nasal tissue were harvested. spnIII allele quantification was performed on DNA extracted from the original inoculum and colonies grown from nasopharynx samples. Allele percentages for original inoculum and bacteria recovered from noses are represented. Statistical analysis was performed using two-way analysis of variance and a Tukey post-comparison test; *P < 0.05.

Article Snippet: The wildtype S. pneumoniae strains used in this study ( ) were provided to us by the German National Reference Center for Streptococci (Aachen) ( ).

Techniques: Infection, Bacteria, Comparison

Journal: Deutsches Ärzteblatt International

Article Title: The Increase in Invasive Bacterial Infections With Respiratory Transmission in Germany, 2022/2023

doi: 10.3238/arztebl.m2023.0261

Figure Lengend Snippet: Invasive isolates (blood culture, sterile aspirate) of S. pneumoniae (n = 12 478), S. pyogenes (n = 6707), H. influenzae (n = 2037), and N. meningitidis (n = 332) per quarter (sampling date), 2017–2023, Germany (ARS, as of 11 April 2023)

Article Snippet: The Institute for Medical Microbiology at the University Hospital, RWTH Aachen University (appointed as National Reference Center [NRC] for streptococci up to the end of 2022) and the NRC for meningococci and H. influenzae receive voluntarily submitted invasive isolates of H. influenzae, N. meningitidis, S. pneumoniae , and S. pyogenes from all routine laboratories in Germany.

Techniques: Sterility, Sampling

Journal: Deutsches Ärzteblatt International

Article Title: The Increase in Invasive Bacterial Infections With Respiratory Transmission in Germany, 2022/2023

doi: 10.3238/arztebl.m2023.0261

Figure Lengend Snippet: Numbers of invasive isolates (blood culture, sterile aspirate) of S. pneumoniae, S. pyogenes, H. influenzae, and N. meningitidis relative to mean seasonal peak values by quarter, 2017–2019*

Article Snippet: The Institute for Medical Microbiology at the University Hospital, RWTH Aachen University (appointed as National Reference Center [NRC] for streptococci up to the end of 2022) and the NRC for meningococci and H. influenzae receive voluntarily submitted invasive isolates of H. influenzae, N. meningitidis, S. pneumoniae , and S. pyogenes from all routine laboratories in Germany.

Techniques: Sterility

Journal: Deutsches Ärzteblatt International

Article Title: The Increase in Invasive Bacterial Infections With Respiratory Transmission in Germany, 2022/2023

doi: 10.3238/arztebl.m2023.0261

Figure Lengend Snippet: Invasive S. pyogenes isolates (blood culture and aspirate, n = 6707) by age group per 100 000 population, per quarter (sampling date), 2017–2023, Germany (ASR, as of 11 April 2023)

Article Snippet: The Institute for Medical Microbiology at the University Hospital, RWTH Aachen University (appointed as National Reference Center [NRC] for streptococci up to the end of 2022) and the NRC for meningococci and H. influenzae receive voluntarily submitted invasive isolates of H. influenzae, N. meningitidis, S. pneumoniae , and S. pyogenes from all routine laboratories in Germany.

Techniques: Sampling

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Ranking of serotype specific macrolide nonsusceptibility among IPD isolates in Germany from 1992 to 2008 (n, overall = 11,807; n, adults = 8,834; n, children = 2,973)

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques:

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Ranking of macrolide nonsusceptibility among IPD isolates in Germany from 1992 to 2008 related to the sampling source (n = 11,807)

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques: Sampling

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Macrolide nonsusceptibilities of IPD isolates in Germany . Macrolide nonsusceptibilities of IPD isolates in Germany (1992 to 2008; n, total = 11,807; n, adults = 8,834; n, children = 2,973; I%, intermediate in percent; R%, resistant in percent; n, number of cases).

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques:

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Serotype specific macrolide nonsusceptibility of IPD isolates in Germany . Serotype specific macrolide nonsusceptibility of IPD isolates in Germany (1992 to 2008; n, serotype 14 = 1,546; n, serotype 6B = 447; n, serotype 19F = 448; n, serotype 19A = 321; n, serotype 9V = 404; n, serotype 23F = 557)

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques:

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Macrolide nonsusceptibility among 7-, 10- and 13-valent vaccine serotypes . Macrolide nonsusceptibility among 7-, 10- and 13-valent vaccine serotypes (IPD isolates in Germany from 1992 to 2008; n, number of cases. Vaccine strains included are: 7-valent: serotypes 4, 6B, 9V, 14, 18C, 19F and 23F; 10-valent: 7-valent serotypes plus 1, 5 and 7F; 13-valent: 10-valent serotypes plus 3, 19A and 6A)

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques:

Journal: BMC Microbiology

Article Title: Macrolide susceptibility and serotype specific macrolide resistance of invasive isolates of Streptococcus pneumoniae in Germany from 1992 to 2008

doi: 10.1186/1471-2180-10-299

Figure Lengend Snippet: Macrolide nonsusceptibility among non 7-, non 10- and non 13-valent vaccine serotypes . Macrolide nonsusceptibility among non 7-, non 10- and non 13-valent vaccine serotypes (IPD isolates in Germany from 1992 to 2008; n, number of cases)

Article Snippet: The aim of this study was to evaluate macrolide susceptibility of all isolates of S. pneumoniae with IPD that were sent to the German National Reference Center for Streptococci (NRCS) between 1992 and 2008 and to evaluate potential trends in nonsusceptibility over time.

Techniques:

Journal: Emerging Infectious Diseases

Article Title: Bacterial Resistance to Penicillin G by Decreased Affinity of Penicillin-Binding Proteins: A Mathematical Model

doi: 10.3201/eid0904.020213

Figure Lengend Snippet: Model structure of the emergence and transmission of penicillin G resistance in Streptococcus pneumoniae and Neisseria meningitidis .

Article Snippet: To validate the predictions of the model for changes in resistance, we used data on S. pneumoniae reported to the French National Reference Center (NRC) for pneumococci ( ).

Techniques: Transmission Assay

Journal: Emerging Infectious Diseases

Article Title: Bacterial Resistance to Penicillin G by Decreased Affinity of Penicillin-Binding Proteins: A Mathematical Model

doi: 10.3201/eid0904.020213

Figure Lengend Snippet: (a) Time to emergence of the first Streptococcus pneumoniae with a given MIC (full line) and time required for 20% of the bacterial population to reach this MIC (dotted line), starting from an all-susceptible pneumococcal population. Error bars correspond to stochastic variations in the model simulations (10th and 90th percentiles based on 100 simulations). (b) Simulated and (c) observed changes with time since 1987 in the distribution of resistance levels in the pneumococcal population in France. Observed data are taken from the Centre National de Référence des Pneumocoques .

Article Snippet: To validate the predictions of the model for changes in resistance, we used data on S. pneumoniae reported to the French National Reference Center (NRC) for pneumococci ( ).

Techniques: